Antibiotics in milk drinkers may cause allergies

National security glass and quartz glass Product Quality Supervision and Inspection Center (SQGTC), recently by the relevant national authority, after re-evaluation, an increase of more glass products Precision bearing inspection items.
National security glass and quartz glass products belonging to the State Quality Supervision and Inspection Center of Quality Supervision, Inspection and Quarantine authorized China National Testing Center National Accreditation Board accredited national testing center. The re-evaluation, the center for the seizure of the glass product range includes: coated glass, hollow glass, float glass, sheet glass, tinted glass, patterned glass, laminated glass, tempered and semi-tempered glass, fire-proof glass, automotive safety glass , aircraft glazing, ship glass, quartz glass, and cast stone products.
National security glass and quartz glass product quality supervision and inspection center dedicated to glass, quartz glass and other products, testing, testing technology research and related standards revision work. Center in 1990, passed the U.S. official agencies AAMVA (American Association of Motor Vehicle Administration) review, as its foreign inspection AIs can be carried out to the American National Standards-based automotive safety glass DOT certification testing.Why milk containing antibiotics?
Antibiotics in milk from cows, dairy cows are injected antibiotics, primarily for the treatment of breast inflammation. Cow mastitis is common, the use of antibiotics is the industry's "open secret." Antibiotics in milk drinkers may cause allergies, or because of prolonged exposure to antibiotics and resistance.
How to achieve "no anti"?
Drug reasonable intervals after milking, milk can be produced without resistance, you can verify by examining the way antibiotics.
An indirect method
Method: the use of β-lactamase hydrolysis of penicillin to the principle of adding a certain amount of milk to penicillin, if present in a concentration of milk β-lactamase, then penicillin by β-lactamase enzyme concentration after will be reduced, in order to determine whether the milk β-lactamase.
Experimental Procedure: Weigh 20 g sample at 4 ℃, 16000rpm was centrifuged 10 min. Take the lower serum 10 g in 50 mL plastic centrifuge tube and placed in plastic centrifuge tubes were incubated at 37 ℃ pot oscillation 30 min. After the incubation tube were added ethanol 15 mL. After 30 min shaking extraction centrifugation, the supernatant was collected on filter paper, pear shaped flask. Concentrated under reduced pressure to evaporate ethanol. After rotary evaporation to Add 10 mL pear-shaped flask of phosphate buffer solution (pH = 8.5), vortex for 1 min pH was adjusted to 8.5. To 1 mL / min rate of the extract through a pretreated Oasis HLB solid phase extraction column with 2 mL of phosphate buffer (pH = 8.5) leaching extraction column, and then 2 mL water rinse. Finally with 3 mL of acetonitrile. The eluate was evaporated to dryness at 40 ℃ with 0.025 M phosphate buffer (pH = 7.0) the residue to constant volume 1 mL, pending the determination on the machine.